Journal: PLoS ONE

Article Title: TACC3 Is Essential for EGF-Mediated EMT in Cervical Cancer

doi: 10.1371/journal.pone.0070353

Figure Lengend Snippet: ( A ) The expression of TACC3 in Ect1/E6E7 (HPV-immortalized ectocervical epithelial), CaSki (HPV-16), C33A (HPV-negative), SiHa (HPV-16) and HeLa (HPV-18) cell lines was determined by western blot analysis. The expression levels were compared to three normal cervix tissues. β-actin was used as a loading control. The intensity of bands was quantified using imageJ software and normalized to β-actin. Data shown are means ± SD of at least three independent experiments. ( B ) Representative immunohistochemical staining on cervical cancer tissue microarray. Quantitative analysis of cervical cancer tissue microarrays showed that the expression of TACC3 is higher in cervical cancer than in normal cervix, but its expression does not correlate with tumor stage ( C ) or grade ( D ). Data shown are means ± SD of at least three independent experiments. *, p <0.05; **, p <0.001.

Article Snippet: Human normal cervix tissue lysates were purchased from Imgenex (San Diego, CA).

Techniques: Expressing, Western Blot, Control, Software, Immunohistochemical staining, Staining, Microarray

Journal: PLoS ONE

Article Title: TACC3 Is Essential for EGF-Mediated EMT in Cervical Cancer

doi: 10.1371/journal.pone.0070353

Figure Lengend Snippet: ( A ) Representative immunohistochemical staining of TACC3 and Snail on cervical cancer tissue microarray. Quantitative analysis of cervical cancer tissue microarrays showed that ( B ) the expression of Snail is higher in cervical cancer than in normal cervix. Data shown are means ± SD of at least three independent experiments. *, p <0.001 ( C ) Snail expression correlates with TACC3 expression (r = 0.80383, p<0.0001 ).

Article Snippet: Human normal cervix tissue lysates were purchased from Imgenex (San Diego, CA).

Techniques: Immunohistochemical staining, Staining, Microarray, Expressing

Journal: Oncotarget

Article Title: Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis

doi: 10.18632/oncotarget.11810

Figure Lengend Snippet: a. HeLa, SiHa, and CaSki cells were stably transfected with control shRNA or Piwil2 shRNA, and cell viability was measured daily. b. Numbers of invading cells in clones stably transfected with control shRNA and Piwil2 shRNA. c. Equal amounts of lysates from cancer cell lines stably transfected with control shRNA or Piwil2 shRNA were separated by SDS-PAGE, and proteins were analyzed by western blotting with specific antibodies against Piwil2 and molecules regulating cell proliferation. d. Tumor growth over time was measured after the subcutaneous injection of 5×10 6 of SiHa cells stably transfected with shPiwil2 control shRNA and Piwil2 shRNA. Tumor volume was monitored by caliper measurements twice weekly, and tumor weight was measured after sacrifice at the end of the experiment. The data are presented as the mean±SD. * P < 0.05 and ** P < 0.01 by Student's t -test.

Article Snippet: To knock down Piwil2 expression, the human cervical cancer cell lines HeLa, SiHa, CaSki, and C33a were transfected in 6-well plates with 1 μg Piwil2 shRNA expression plasmids (TG302470, Origene, Beijing), which contained four unique 29-mer shRNA constructs, respectively (1.

Techniques: Stable Transfection, Transfection, shRNA, Clone Assay, SDS Page, Western Blot, Injection

Journal: Scientific Reports

Article Title: On the mechanism of miR-29b enhancement of etoposide toxicity in vitro

doi: 10.1038/s41598-024-70856-y

Figure Lengend Snippet: Effect of siRNA against Mcl-1 on etoposide cytotoxicity in different cell lines. (Panel a ): The cells were transfected (6 h) with siRNA against Mcl-1 or negative control. Cells were then treated with 60 μM etoposide 24 h post transfection and incubation continued until experiment was ended. The proliferation was calculated from doubling time value as explained in Materials and methods section. Each bar represents mean ± s.d. of at least three independent experiments (N HeLa = 4, N Hep G2 = 3, N Caco-2 = 3), in each experiment triplicate measurements were performed. **p < 0.01 versus corresponding negative control. (Panel b ): Evaluation of siRNA efficiency by western blot analysis in Hela cells. The cells were transfected (6 h) with anti-Mcl-1 siRNA or negative control. Shown are representative western blots of cells cultured for 24 h after transfection. (Panel c ): Evaluation of siRNA efficiency by western blot analysis in Hep G2 cells. Treatment was the same as for panel b. Panel d: Evaluation of siRNA efficiency by western blot analysis in Caco-2 cells. Treatment was the same as for (panel b ).

Article Snippet: HeLa cells were obtained from The European Collection of Authenticated Cell Cultures (ECACC, No. 93021013 Human Negroid cervix epitheloid carcinoma cell line) via Sigma-Aldrich as provider.

Techniques: Transfection, Negative Control, Incubation, Western Blot, Cell Culture